TALLAHASSEE, Fla. (AP) — Florida’s Republican governor signed a bill Tuesday barring transgender females from playing on public school teams intended for student athletes born as girls, plunging the state into the national culture war over transgender rights.

“In Florida, girls are going to play girls sports and boys are going to play boys sports,” Gov. Ron DeSantis said as he signed the bill into law at a private Christian academy in Jacksonville that would not be subject to the law. “We’re going to make sure that that’s the reality.”

The new law, sure to be challenged as unconstitutional, inflames an already contentious discussion unfolding nationally as Republican-controlled states move to limit the rights of LGBTQ people, whose advocates were particularly annoyed that the legislation was signed on the first day of Gay Pride Month.

The NCAA, which oversees college athletics, has said it has “a long-standing policy that provides a more inclusive path for transgender participation in college sports.” The NCAA currently requires transgender women to get treatment to lower their testosterone levels before they can compete in women’s sports.

For more on Gov. DeSantis, check out Shannon Longworth’s story here.